Interleukin 6 (IL-6)
IL-6 Antibody
| Name |
Anti-Human Interleukin 6 (IL-6) antibody |
| Catalog # |
R270c7 |
R233a3 |
R231n3 |
R269t9 |
R449k7 |
R448a3 |
| Source |
Mouse monoclonal antibody, cultured in vitro |
Rabbit monoclonal antibody, cultured in vitro |
Platforms
Pairs |
Chemiluminescence immunoassay (CLIA) |
Immunochromatography assay (ICA) |
| R270c7 (Capture) -R269t9 (Detection) |
R231n3 (Capture) -R233a3 (Detection)
R449k7 (Capture) -R448a3 (Detection) |
Buffer |
1xPBS，pH 7.4 |
| Purity |
Purity>95%, purified by Protein A/G chromatography |
| Storage |
Aliquot and store at -20°C or lower. Avoid freeze / thaw cycles. |
IL-6 Antibody Conjugates
IL-6 Antibody Conjugated Magnetic Beads
| Name |
Catalog # |
| IL-6 antibody conjugated magnetic beads |
B140n1 |
| B141n1 |
| B142n1 |
Labeled PCT Antibody
| Name |
Catalog # |
| Biotin labeled PCT antibody |
L153o1 |
| Acridinium ester (AE) labeled PCT antibody |
L150o1 |
| Alkaline phosphatase (ALP) labeled PCT antibody |
L152o1 |
IL-6 Antigen
| Name |
Interleukin 6 (IL-6) antigen |
| Description |
Recombinant, C-terminal His-tagged, in vitro expressed from mammalian cells |
| Applications |
Calibrator and quality control product |
| Catalog # |
K1511 |
| Purity |
>90%, analyzed by R250-stained SDS-PAGE |
| Buffer |
1x PBS，pH 7.4 |
| Storage |
Aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| SDS-PAGE |
Predicted MW around 21.6kDa
 |
Product Information
Alkaline phosphatase-labeled Chemiluminescence (ALP-CLIA) platform
Correlation in clinical samples
R270c7-R269t9 matched antibody pair was verified in RocheTM serum assigned samples (40 cases, concentration:1.5-4525pg/mL, clinical coefficient of determination R2 > 0.99).
| Sample No. |
Concentration
（pg/mL） |
Concentration of Detection
（pg/mL） |
| 1 |
1.50 |
1.30 |
| 2 |
1.50 |
1.40 |
| 3 |
2.61 |
2.77 |
| ... |
... |
... |
| 39 |
4471 |
4033 |
| 40 |
4525 |
4311 |
Fig.1 Correlation of IL-6 on CLIA platform in clinical samples
Table.1 Correlation data in clinical samples
Diagnostic Significance of Interleukin 6 (IL-6)
Biological Function, Signal Transduction and Clinical Application of Interleukin-6 (IL-6)
Interleukin 6 (IL-6) is a sensitive indicator for diagnosis of early stage acute infection. It is widely applied in infectious diseases, tumors, arthritis, physical trauma, cardiovascular diseases, renal diseases etc.
The normal concentration of IL-6 in the human body is less than 7pg/mL, and the corresponding relationship between the content of IL-6 and the diagnosis of inflammatory diseases is as follows:
- IL -6 concentration<7pg/mL: Normal
- IL-6 concentration 7-150pg/mL: Presence of mild inflammation or infection;
- IL-6 concentration 150-250pg/mL: General bacterial infection or systemic inflammatory response
- IL-6 concentration >250pg/mL: Suspected sepsis
IL-6 is a polypeptide substance secreted by monocytes, macrophages, T cells, B cells and other cells. It is a kind of cytokine with multiple biological activities such as promoting differentiation and growth of cells. It does not only act on the immune system itself, but also widely act on neural, endocrine, cardiovascular and other systems. IL-6 is also an important cytokine involved in various adjustments of the body. IL-6 plays a core role in regulating the inflammatory immune response and is an important mediator. At early stages of infectious inflammation, monocytes and macrophages produce acute IL-6 while stimulated by Toll-like receptors, and the content of IL-6 in the body increases. Such acute IL-6 will strongly induce the expression of acutephasereactants (APR) such as C-reactive Protein, Serum amyloid A, etc. and reduce the expression of albumin, transferrin, etc. to act its regulatory effects.
Related Products
Related Articles
